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Image Search Results
Journal: International journal of molecular sciences
Article Title: Optimization of Mass Spectrometry Imaging for Drug Metabolism and Distribution Studies in the Zebrafish Larvae Model: A Case Study with the Opioid Antagonist Naloxone.
doi: 10.3390/ijms241210076
Figure Lengend Snippet: Figure 7. Comparison of the spatial distribution of naloxone (sodium adduct ion (m/z 350.136) and potassium adduct ion (m/z 366.110)) inside ZF larval whole body between (a,b) the protocol used in the previous studies [26,27] and (c,d) the optimal condition investigated in this study. All MS images were visualized with a three-dimensional view function in SCiLS Lab version 2022a Premium 3D software, based on the diagram of a ZF larva shown in Figure 4. The experimental conditions were the following: (a,b) 14 passes of spraying matrix deposition using 15 mg mL−1 DHB containing 0.1% TFA and 40% gelatin embedding medium, a total of 10 slides for one respective larva; (c,d) 8 passes of spraying matrix deposition using 5 mg mL−1 CHCA containing 0.1% TFA and 40% gelatin embedding medium, a total of 11 slides for one respective larva. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option, and then they were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.
Article Snippet: 2.2.0, flexImaging version 5.0, and
Techniques: Comparison, Software, Generated, Concentration Assay
Journal: International journal of molecular sciences
Article Title: Optimization of Mass Spectrometry Imaging for Drug Metabolism and Distribution Studies in the Zebrafish Larvae Model: A Case Study with the Opioid Antagonist Naloxone.
doi: 10.3390/ijms241210076
Figure Lengend Snippet: Figure 8. MALDI-MS images of the most abundant ZF metabolites of naloxone (M2 (m/z 326.078), M4 (m/z 368.125), M7 (m/z 542.142), M10/M14 (m/z 382.105), and M35 (m/z 308.068) potassium adduct ions) detected inside ZF larvae (whole body) prepared under the optimal conditions, as described above. All MS images were visualized with a three-dimensional view function in SCiLS Lab version Premium 2022a 3D software, based on the diagram of a ZF larva shown in Figure 4. The images were generated by preparing a color map from blue (no detection) to yellow (high local concentration) with a 50% transparency option and then were further processed in 96 dpi resolution with 24-bit color under a denoising state. The red lines indicate individual larval body sections that were overlapped for 3D visualization, and approximately 15 sections were usually utilized for this visualization.
Article Snippet: 2.2.0, flexImaging version 5.0, and
Techniques: Software, Generated, Concentration Assay